nico λde3 (New England Biolabs)

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New England Biolabs nico λde3
Nico λde3, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 467 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nico+%CE%BBde3/NiCo21(DE3)+Competent+E%2E+coli/bio_rxiv__2023__10__17__562781-105-28-31
Average 97 stars, based on 467 article reviews

nico λde3 - by Bioz Stars, 2026-10

97/100 stars

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Inverse PCR:

Article Title: Type II restriction of 2-aminoadenine (dZ) modified DNA and production of dZ-modified plasmid in E. coli
Article Snippet: .. The dZ-modified pUC19 and regular pUC19 inverse PCR DNA were transferred into E. coli lab strains by transformation using 20 ng DNA: NEB Turbo (Mrr + , E. coli K strain), C2925 (Mrr + , Dam - , Dcm - , McrA - , McrB - ), NEB 10β (Mrr - , RecA - ), C2683 (Mrr - , McrBC - , K strain), T7 shuffle (C3026, K strain), Nico (λDE3) (C2529, B strain), NEB Express (C2523, B strain). .. Less than 2-fold differences were observed in transformation efficiency when the dZ-modified pUC19 PCR DNA was transferred into the above strains compared to unmodified inverse PCR DNA (data not shown), with the exception of two strains, NEB Turbo and C2925.

Transformation Assay:

Expressing:

Article Title: Expression of Human ACE2 N-terminal Domain, Part of the Receptor for SARS-CoV-2, in Fusion With Maltose-Binding Protein, E. coli Ribonuclease I and Human RNase A.
Article Snippet: .. The protein expression level was evaluated in four T7 expression strains: T7 Express (C2566, NEB), T7 Express with LysY and lacIq (C3013, NEB), T7 SHuffle (C3026, E. coli K strain, NEB), and Nico (λDE3) with reduced histidine-rich background proteins (NEB). ..

Produced:

Article Title: Type II restriction of 2-aminoadenine (dZ) modified DNA and production of dZ-modified plasmid in E. coli
Article Snippet: The T7 expression vector pET21b with C-terminal 6xHis tag was originally purchased from Novagen (NdeI-XhoI). .. E. coli competent cells of the following strains were produced in-house at NEB: NEB Turbo (Mrr + , E. coli K strain), C2925 (Mrr + , Dam - , Dcm - , McrA - , McrB - ), NEB 10β (RecA - ), C2683 (Mrr - , McrBC - , K strain), T7 shuffle (C3026, K strain), Nico (λDE3) (C2529, B strain), NEB Express (C2523, B strain). ..

Cloning:

Article Title: Type II restriction of 2-aminoadenine (dZ) modified DNA and production of dZ-modified plasmid in E. coli
Article Snippet: .. For cloning and working with dZ-modified DNA, it is recommended to use Mrr-deficient E. coli strains: NEB 10β, C2683 (Mrr - , McrBC - ), T7 shuffle (C3026), Nico (λDE3) (C2529), NEB Express (C2523), C2566 (T7 Express). ..

Review